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  • Structure-Function Relationships in Temperature Effects on Bacterial . . .
    The results of our study indicate that the “fast” P leiognathi luciferase is thermolabile and has maximal activity in a narrower temperature range than does the “slow” V harveyi luciferase The latter is weakly sensitive to temperature change and remains active up to 35 °C
  • Kinetics of the thermal inactivation and the refolding of bacterial . . .
    subtilis cells with thermal inactivated luciferases from P leiognathi (A) and P luminescens (B), which regained their activity after cell cultures were moved to room temperature Luciferases were thermal inactivated in vivo by exposure of carrier cells at either 47°С (E coli) or 51°С (B subtilis) Relative luminescence shown on vertical
  • Photobacterium leiognathi - an overview | ScienceDirect Topics
    P leiognathi subsp leiognathi shows higher light intensities at low salt concentrations and a more intense blue color, while P leiognathi subsp mandapamensis shows higher light intensities at high salt concentrations and blue-green color
  • Ketones 2-heptanone, 2-nonanone, and 2-undecanone inhibit . . . - Springer
    Here, we show that such VOCs as ketones 2-heptanone, 2-nonanone, and 2-undecanone inhibit the DnaKJE-ClpB bichaperone de-pendent refolding of heat-inactivated bacterial luciferases The inhibitory activity of ketones had highest effect in Escherichia coli ibpB::kan cells lacking small chaperone IbpB
  • Rate constants (K t ) of P. leiognathi luciferase . . . - ResearchGate
    When heat shock is caused by ethanol addition, transcription of lux-operon increases only in the natural strain of P temperata, but not in the heterologous system of E coli cells
  • Re: How does heat shock work (in bacterial transformation)?
    A simple interpretation of these observations is that heat-shock makes the cells leaky Pores formed in the outer membrane permit DNA to enter and the loss of membrane potential helps the negatively-charged DNA to cross the inner membrane
  • 2. Photobacterium leiognathi - Microbe Whisperer
    Since P Leiognathi needs a quorum (a quota of cells) to form this light, the pony fish gives P Leiognathi a custom-designed nook inside its body for its microbial “shadchanits”, filling the specialized organ to the brim with these blue-green microscopic pearls of bioluminescence
  • Kinetics of the thermal inactivation and the refolding of bacterial . . .
    The kinetics of the thermal inactivation and the refolding of luciferases from Photorhabdus luminescens and Photobacterium leiognathi were compared in Gram negative and Gram positive bacteria In B subtilis cells, these luciferases are substantially more thermostable than in Escherichia coli
  • Structure-Function Relationships in Temperature Effects on . . . - MDPI
    The results of our study indicate that the “fast” P leiognathi luciferase is thermolabile and has maximal activity in a narrower temperature range than does the “slow” V harveyi luciferase The latter is weakly sensitive to temperature change and remains active up to 35 °C
  • A calorimetric investigation of the growth of the luminescent bacteria . . .
    bacterium leiognathi These experiments were performed with a Tronac isothermal microcalorimeter modified with a fiber optic light guide to allow in situ detection of light Escherichia coli and a dark variant of P leiognathi were also examined to provide points ofreference It is demonstrated that B harveyi seems to pause in the





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